The final stage of a plant genetic engineering project often takes the researcher into the greenhouse. At this point, the research adds the isolated gene to plants and determines if the gene alters phenotype.

What you'll learn in this topic

• how a gene can be inserted into a plant genome by transformation with Agrobacterium and the Ti plasmid.
• how selection is used to screen for plant cells that have been transformed
• how transgenic plants are created from a clump of cells
• how to determine if the added gene affects phenotype

Your task is to carry out a series of steps that will create a transformed plant from the gene you amplified using PCR. You will then determine if transformed plants have a high vitamin E phenotype.